Table 4.
Effect of different compounds on ADPG hydrolysis catalyzed by AGPPase
| Effectors | Ki, mM | Type of inhibition |
|---|---|---|
| Glucose, 100 mM | — | ND |
| Adenine, 5 mM | — | ND |
| Adenosine, 5 mM | — | ND |
| Metal ions: Co2+, Ca2+, Mg2+, and Mn2+, 1 mM | — | ND |
| NAD+ | 3.28 | Competitive |
| Phosphorylated compounds | ||
| Pi | 0.39 | Competitive |
| PPi | 0.82 | Competitive |
| 3-PGA | 1.21 | Competitive |
| ATP | 0.89 | Competitive |
| ADP | 0.43 | Competitive |
| AMP | 0.74 | Competitive |
| NADP+ | 0.67 | Competitive |
| Phosphodiesterase inhibitors | ||
| l-Cysteine, 5 mM | — | ND |
| Glutamate, 5 mM | — | ND |
| Reduced glutathione, 5 mM | — | ND |
| Aspartate, 5 mM | — | ND |
| Ascorbate, 1 mM | — | ND |
| Fluoride, 1 mM | — | ND |
| EDTA, 1 mM | — | ND |
| β-Mercaptoethanol, 1 mM | — | ND |
| DTT, 1 mM | — | ND |
Kinetic parameters (Km, Vmax, Ki, and stoichiometry) obtained are the mean values from five independent experiments. ND, not detectable.