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. 2000 Jul 11;97(15):8705–8710. doi: 10.1073/pnas.120168097

Table 5.

Subcellular localization of AGPPase in cultured sycamore cells

Enzyme Protopast lysate activity, milliunits/mg of protein Stepped Percoll gradient centrifugation
Amyloplast activity, milliunits/mg of protein Amyloplast as % of lysate Recovery range, %
ADH 2563  ± 1331 0.13  ± 0.03 0.006  ± 0.01 110  ± 31
α-Mannosidase 325  ± 63 0.13  ± 0.09 0.042  ± 0.02 83  ± 26
PPase 4356  ± 1040 43.7  ± 8.71 1.0  ± 0.20 82  ± 14
AGPPase 16.5  ± 7.2 0.17  ± 0.06 1.0  ± 0.17 120  ± 39

 Data are presented as the mean ± SD obtained from six separate preparations.