Table 5.
Enzyme | Protopast lysate activity, milliunits/mg of protein | Stepped Percoll gradient centrifugation
|
||
---|---|---|---|---|
Amyloplast activity, milliunits/mg of protein | Amyloplast as % of lysate | Recovery range, % | ||
ADH | 2563 ± 1331 | 0.13 ± 0.03 | 0.006 ± 0.01 | 110 ± 31 |
α-Mannosidase | 325 ± 63 | 0.13 ± 0.09 | 0.042 ± 0.02 | 83 ± 26 |
PPase | 4356 ± 1040 | 43.7 ± 8.71 | 1.0 ± 0.20 | 82 ± 14 |
AGPPase | 16.5 ± 7.2 | 0.17 ± 0.06 | 1.0 ± 0.17 | 120 ± 39 |
Data are presented as the mean ± SD obtained from six separate preparations.