Table 6.

Subcellular localization of AGPPase in barley endosperm

Enzyme	Endosperm activity, milliunits/mg of protein	Nycodenz density gradient
		Amyloplast activity, milliunits/mg of protein	Amyloplast as % of endosperm	Recovery range, %
ADH	3.61  ± 0.4	0.024  ± 0.0026	0.67  ± 0.07	95  ± 15
α-Mannosidase	2.48  ± 0.5	0.002  ± 0.0001	0.08  ± 0.01	101  ± 16
PPase	78.5   ± 1.6	5.98   ± 0.27	7.65  ± 0.50	98  ± 26
AGPPase	18.9   ± 0.8	0.55   ± 0.17	2.91  ± 0.47	112  ±  8

 Data are presented as the mean ± SD obtained from six separate preparations.