Skip to main content
. Author manuscript; available in PMC: 2010 Mar 24.
Published in final edited form as: Biochemistry. 2009 Mar 24;48(11):2522–2528. doi: 10.1021/bi801686b

Figure 2. Solubility of LC/A4.

Figure 2

(A) Kyte and Doolittle mean hydrophobicity profile of LC/A1-4. Arrow indicates residues substitutions causing a calculated increase in hydrophobicity locally: LC/A1, LC/A2, LC/A3 (—), and LC/A4 (---). Alignment and hydrophobicity plot calculated using BioEdit. LC/A1-A3 were plotted as solid lines due to their overlapping sequence. (B) LC/A subtype alignment of residues within solubility pocket of LC/A4 (260, 264). Underlined lettering indicates residue substitution. (C) Quantification of LC/A4 and LC/A4-I264R solubility upon induction, lysis, and centrifugation as measured by densitometry of a Coomassie-stained, 12% SDS-PAGE gel.