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. Author manuscript; available in PMC: 2009 Jun 23.
Published in final edited form as: J Immunol. 2009 Jan 15;182(2):759–765. doi: 10.4049/jimmunol.182.2.759

FIGURE 5.

FIGURE 5

IL-7R+ pre-DP thymocytes are impaired in maturation to the DP stage. A, Schematic of experimental design to determine the developmental potential of sorted pre-DP thymocytes in vitro. B, IL-7R+ pre-DP thymocytes fail to mature to the DP stage. Sorted IL-7R+ and IL-7R- pre-DP cells from both C57BL/6 and CAT-Tg mice were cultured in vitro for 20–24 h. Representative dot plots show expression of CD4 and CD8 following in vitro culture. C, Statistically significant number of IL-7R+ pre-DP cells fail to mature to the DP stage. Quantification of the fraction of cells that remain DN after culture in B. Data in the graph are average of three mice of each genotype in a representative experiment.