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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Apr 17;237(3):258–266. doi: 10.1016/j.taap.2009.04.008

Figure 5. Cr(VI) modulated the levels of histone methyltransferase G9a.

Figure 5

(A) Cr(VI) exposure increased G9a protein levels. A549 cells were treated with 5 or 10 μM of Cr(VI) for 24 hours. Total protein lysates were extracted and analyzed with antibody against G9a. Antibody against tubulin was used to assess the loading of proteins. (B) A549 cells were exposed to 5 or 10 μM Cr(VI) for 24 hours. The global levels of H3K9me2 were measured using specific antibodies. Coomassie blue staining was used to assess the equal loading of the histones. (C and D) Chromate exposure increased G9a mRNA levels after 24 hr exposure. A549 cells were treated with 5 or 10 μM Cr(VI) for 1 (D) or 24 (C) hours. G9a mRNA levels were analyzed by Northern blotting. The ethidium bromide staining of 28S and 18S RNA was performed to assess the loading of RNA samples. The relative intensity of the bands was measured and plotted as the mean ratio of G9a mRNA to 18S RNA ± SE (error bars). * P < 0.05, ** P < 0.01, *** P < 0.001.