Fig. 2.

Loss of osteogenic differentiation and BMP signal integration by HTY mutant RUNX2 protein. a Each TBRE element contains a Smad and an overlapping Runx DNA-binding motif. Cells were cotransfected as described and treated with 100 ng BMP2 and luciferase activity was determined. b Commitment of reconstituted Runx2 null cells to osteoblast lineages was tested by ALP expression. Adenovirus-infected cells were cultured in the presence of BMP2 and relative expression levels of ALP marker gene were monitored by real-time RT-PCR analysis.