Table 1.
Treatment | cfu/g (× 106)*
|
cfu/g (× 108)*
|
||||||
---|---|---|---|---|---|---|---|---|
Exp. 1
|
Exp. 2
|
Exp. 3
|
Exp. 4
|
|||||
Col-0 | Col-0 | Col-0 | cpr1 | jar1 | etr1 | NahG | npr1 | |
Ctrl | 24 ± 4a | 17 ± 2a | 122 ± 17a | 30 ± 2a | 138 ± 6b | 116 ± 31a | 156 ± 22a | 72 ± 9a |
WCS417r | 15 ± 2b | 10 ± 2b | 55 ± 3b | 22 ± 2b | 181 ± 21a | 112 ± 5a | 81 ± 9b | 80 ± 12a |
Pst(avrRpt2) | 11 ± 1b | 31 ± 3c | 54 ± 3b | 173 ± 48a | 68 ± 28a | |||
Pst(avrRpt2) + WCS417r | 6 ± 2c | 34 ± 5c | 60 ± 19b | 73 ± 7b | 68 ± 10a | |||
SA | 9 ± 2b | |||||||
SA + WCS417r | 5 ± 1b |
Values presented are average numbers (± SE) of cfu/g fresh weight, each from three sets of six whole shoots harvested 4 days after challenge inoculation with virulent Pst, and correspond to the bioassays shown in Fig. 1A (Exp. 1), Fig. 1B (Exp. 2), Fig. 1C (Exp. 3), and Fig. 2 (Exp. 4). The number of Pst bacteria present in the leaves just after challenge ranged from 2 × 105 to 106 cfu/g but was similar for all treatments within single experiments. Within each genotype, different letters indicate statistically significant differences between treatments (Fisher's Least Significant Differences test, α = 0.05). Similar results were obtained in repeated experiments, except for the difference between control- and WCS417r-treated jar1 plants, which was not apparent in the repeats nor in similar experiments in other studies (35).