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(A) The membrane charge density σM of DOTAP/DOPC/cholesterol–DNA complexes: The squares show σM obtained experimentally from X-ray diffraction data, using Eq.1 and the values of dDNA shown in Fig. 2 B. The triangles show σM as calculated according to Eq.2, taking membrane saturation with cholesterol at Φcholesterol = 0.4 into account as described in the text. (B) The ratio r = ADOTAP/ADOPC, plotted against Φcholesterol, illustrates the variation in ADOTAP with increasing Φcholesterol.
