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FISH genotyping of inversion polymorphisms. (A) Metaphase FISH validation of the 8p23 inversion using two probes located inside of the inversion. Metaphase FISH-based assay to resolve inversions <2 Mb using one probe located inside and one outside the inverted region is shown for the 15q13.3 (B), 17q12 (C) and 3q29 (D) inversions. Interphase triple color FISH validation was used for the 15q24 inversion (E). Arrows indicate inverted chromosomes.
