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. Author manuscript; available in PMC: 2009 Jun 24.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2004 Jun 24;287(5):G943–G953. doi: 10.1152/ajpgi.00121.2004

Fig. 6.

Fig. 6

Effect of HNF-1α, HNF-1β, and cdx-2 expression on calbindin D9k promoter activity in intestinal (TC7) and nonintestinal (HeLa) cells. Luciferase reporter constructs containing CaBP4600 were cotransfected into 50% confluent HeLa (A) or TC7 (B) cells with control vector (250 ng of pCB6), mouse cdx-2 expression vector (250 ng of pCB6-mcdx-2), mouse HNF-1α expression vector (250 ng of pBJ5-HNF-1α), or both cdx-2 and HNF-1α expression vector (250 ng of each). C: 50% confluent cultures of TC7 cells were transfected with CaBP4600 along with control vector, HNF-1α expression vector (0.05 µg), HNF-1β expression vector (0.1 µg), or HNF-1α expression vector mixed with increasing amounts of HNF-1β expression vector (0.05, 0.1, or 0.2 µg). Promoter activities were assessed 24 h after transfection and are expressed relative to the induction seen in the control vector transfected cells (relative value = 1). In A and B, each bar represents the means ± SE (n = 6). In C, each bar represents the means ± SE (n = 3). Bars with different letters are significantly different from one another (Fishers least significant difference test, P < 0.05).