Figure 6.

TAT-STEP increases SE-induced cell death and decreases immediate-early gene expression in the cortex. In A–C, His-tagged TAT-STEP was microinjected into the motor cortex via an indwelling guide cannula. SE was induced 2 h later, and mice were killed 6 h after SE onset. A, TAT-STEP was visualized with an anti-His antibody, followed by Alexa 488-conjugated secondary antibody (color-coded red for clarity). The high-magnification image (taken from the boxed region in the left panel) identifies individual cells that had taken up TAT-STEP. B, Cell death was determined using Fluoro-Jade B staining. Compared with the contralateral hemisphere or with the injection of TAT-myc, TAT-STEP increased SE-induced cell death. In saline (Sal)-injected mice, TAT-STEP did not induce cell death. C, Double labeling for Fluoro-Jade B (FJB; left) and TAT-STEP (anti-His; middle) revealed that SE-induced death occurred in TAT-STEP-expressing cells. D, Representative data showing that SE-induced JunB expression was decreased in the TAT-STEP-infused hemisphere compared with the contralateral hemisphere or to TAT-myc infusion. Animals were killed 2 h after SE onset. Scale bars: A, 500 μm (low-magnification image) and 50 μm (high-magnification image); B, 200 μm (low-magnification image) and 50 μm (high-magnification image); B, 10 μm; C, 10 μm; D, 50 μm.