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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Oncogene. 2009 Mar 23;28(16):1864–1874. doi: 10.1038/onc.2009.35

Figure 2. The enhanced sensitivity of WT7 cells to death-inducing stimuli can be reversed by inhibiting pVHL expression.

Figure 2

(A) pVHL levels in whole cell lysates from PRC2 cells, WT7 cells, and WT7 cells stably expressing shRNA targeting VHL (WT7-shVHL) or a non-targeting control (WT7-shNT) were analyzed by immunoblotting. Tubulin was immumoblotted as a control for protein loading. Similar results were obtained in 2 additional experiments (e.g., see figure 3C). (B) PRC2, WT7, WT7-shNT and WT7-shVHL cells were treated with etoposide (100 μM) or left untreated for 24 h. After fixing the cells and staining with Hoechst dye, the percentage of viable cells was determined as described in figure 1A and in Materials and methods. (C) RCC cells were exposed to UV radiation (1000 J/m2) or left untreated and 24 h later the percentage of viable cells was determined. Results in B and C represent the mean ± SEM from 3 independent experiments (#, p<0.05 compared with PRC2 cells; *, p<0.05 compared with WT7 and WT7-shNT cells).