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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Cancer Cell. 2009 Feb 3;15(2):148–159. doi: 10.1016/j.ccr.2008.12.017

Figure 1. PC-3 prostate cancer cells require Rictor to form tumors as xenografts.

Figure 1

(A) Generation of PC-3 cells with stable knockdown of Rictor. Using a lentiviral delivery system, two independent shRNAs (shRictor1 or shRictor2) that silence Rictor expression or a control shRNA targeting luciferase (shLuc) were stably expressed in PC-3 cells. Protein lysates prepared 6 days post-infection were probed with the indicated antibodies. (B) Rictor knockdown impairs cell proliferation in vitro. PC-3 cells stably expressing shLuc, shRictor1 or shRictor2 were seeded in triplicate at equal density and cell number was counted on four consecutive days. Fold change in cell number is shown. (C) PC-3 cells require mTORC2 to form tumors as xenogfrafts. PC-3 cells stably expressing shLuc, shRictor1 or shRictor2 were injected subcutaneously into nude mice. Tumors were dissected and photographed 28 days post injection. Scale bar = 2mm (D) Average tumor volume (mm3) and SEM is plotted.