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. 1991 Jul;29(7):1486–1492. doi: 10.1128/jcm.29.7.1486-1492.1991

Characterization of serogroup A and B strains of Neisseria meningitidis with serotype 4 and 21 monoclonal antibodies and by multilocus enzyme electrophoresis.

E Wedege 1, D A Caugant 1, L O Frøholm 1, W D Zollinger 1
PMCID: PMC270139  PMID: 1909347

Abstract

The reactions of serogroup A strains of Neisseria meningitidis with one monoclonal antibody specific for serotype 21 and three different monoclonal antibodies specific for serotype 4 were compared with those of serogroup B strains previously assigned to serotype 4. Antibody binding was studied by enzyme-linked immunosorbent assay (ELISA), dot blotting, and immunoblotting. Characterization of the isolates by the electrophoretic mobilities of 14 metabolic enzymes showed 50 multilocus enzyme genotypes. All except two genotypes fell into three distinct clusters: I, IIa and IIb. The enzyme genotypes of serogroup B strains were mainly in cluster I, and 88% of the serogroup A strains had genotypes in clusters IIa and IIb. Serogroup B strains generally reacted with all three serotype 4 monoclonal antibodies in ELISA and dot blotting but with only two in immunoblots. Serogroup A strains showed two different reactions in the blotting methods: either binding of the serotype 21 antibody only or binding of this and two of the three serotype 4 monoclonal antibodies. Strains of the first pattern were in clusters I and IIa, whereas all but two strains in cluster IIb were of the second pattern. In ELISA, an additional reaction of two of the serotype 4 monoclonal antibodies with serogroup A isolates was observed. The different binding of these two monoclonal antibodies in ELISA and the blotting methods appeared to result from heat inactivation of the meningococcal cells and use of detergent-containing reagents in ELISA. The results show that the serotype of serogroup A strains is distinct from serotype 4 of serogroup B strains.

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Selected References

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  1. Abdillahi H., Crowe B. A., Achtman M., Poolman J. T. Two monoclonal antibodies specific for serotype 4 antigen of Neisseria meningitidis. Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):293–296. doi: 10.1007/BF01963105. [DOI] [PubMed] [Google Scholar]
  2. Abdillahi H., Poolman J. T. Typing of group-B Neisseria meningitidis with monoclonal antibodies in the whole-cell ELISA. J Med Microbiol. 1988 Jul;26(3):177–180. [PubMed] [Google Scholar]
  3. Caugant D. A., Frøholm L. O., Bøvre K., Holten E., Frasch C. E., Mocca L. F., Zollinger W. D., Selander R. K. Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4927–4931. doi: 10.1073/pnas.83.13.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caugant D. A., Mocca L. F., Frasch C. E., Frøholm L. O., Zollinger W. D., Selander R. K. Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. J Bacteriol. 1987 Jun;169(6):2781–2792. doi: 10.1128/jb.169.6.2781-2792.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crowe B. A., Abdillahi H., Poolman J. T., Achtman M. Correlation of serological typing and clonal typing methods for Neisseria meningitidis sero-group A. J Med Microbiol. 1988 Jul;26(3):183–187. [PubMed] [Google Scholar]
  6. Crowe B. A., Wall R. A., Kusecek B., Neumann B., Olyhoek T., Abdillahi H., Hassan-King M., Greenwood B. M., Poolman J. T., Achtman M. Clonal and variable properties of Neisseria meningitidis isolated from cases and carriers during and after an epidemic in The Gambia, West Africa. J Infect Dis. 1989 Apr;159(4):686–700. doi: 10.1093/infdis/159.4.686. [DOI] [PubMed] [Google Scholar]
  7. Frasch C. E., Zollinger W. D., Poolman J. T. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev Infect Dis. 1985 Jul-Aug;7(4):504–510. doi: 10.1093/clinids/7.4.504. [DOI] [PubMed] [Google Scholar]
  8. Friguet B., Djavadi-Ohaniance L., Goldberg M. E. Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen. Mol Immunol. 1984 Jul;21(7):673–677. doi: 10.1016/0161-5890(84)90053-1. [DOI] [PubMed] [Google Scholar]
  9. Hollander Z., Katchalski-Katzir E. Use of monoclonal antibodies to detect conformational alterations in lactate dehydrogenase isoenzyme 5 on heat denaturation and on adsorption to polystyrene plates. Mol Immunol. 1986 Sep;23(9):927–933. doi: 10.1016/0161-5890(86)90122-7. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Miller K. F., Bolt D. J., Goldsby R. A. A rapid solution-phase screening technique for hybridoma culture supernatants using radiolabeled antigen and a solid-phase immunoadsorbent. J Immunol Methods. 1983 May 13;59(3):277–280. doi: 10.1016/0022-1759(83)90188-6. [DOI] [PubMed] [Google Scholar]
  12. Mocca L. F., Frasch C. E. Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. J Clin Microbiol. 1982 Aug;16(2):240–244. doi: 10.1128/jcm.16.2.240-244.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olyhoek T., Crowe B. A., Achtman M. Clonal population structure of Neisseria meningitidis serogroup A isolated from epidemics and pandemics between 1915 and 1983. Rev Infect Dis. 1987 Jul-Aug;9(4):665–692. doi: 10.1093/clinids/9.4.665. [DOI] [PubMed] [Google Scholar]
  14. Poolman J. T., Abdillahi H. Outer membrane protein serosubtyping of Neisseria meningitidis. Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):291–292. doi: 10.1007/BF01963104. [DOI] [PubMed] [Google Scholar]
  15. Poolman J. T., de Marie S., Zanen H. C. Variability of low-molecular-weight, heat-modifiable outer membrane proteins of Neisseria meningitidis. Infect Immun. 1980 Dec;30(3):642–648. doi: 10.1128/iai.30.3.642-648.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosenqvist E., Wedege E., Høiby E. A., Frøholm L. O. Serogroup determination of Neisseria meningitidis by whole-cell ELISA, dot-blotting and agglutination. APMIS. 1990 Jun;98(6):501–506. [PubMed] [Google Scholar]
  17. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sippel J. E., Quan A. Homogeneity of protein serotype antigens in Neisseria meningitidis group A. Infect Immun. 1977 May;16(2):623–627. doi: 10.1128/iai.16.2.623-627.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wedege E., Bryn K., Frøholm L. O. Restoration of antibody binding to blotted meningococcal outer membrane proteins using various detergents. J Immunol Methods. 1988 Oct 4;113(1):51–59. doi: 10.1016/0022-1759(88)90381-x. [DOI] [PubMed] [Google Scholar]
  21. Wedege E., Frøholm L. O. Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting. Infect Immun. 1986 Feb;51(2):571–578. doi: 10.1128/iai.51.2.571-578.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wedege E., Høiby E. A., Rosenqvist E., Frøholm L. O. Serotyping and subtyping of Neisseria meningitidis isolates by co-agglutination, dot-blotting and ELISA. J Med Microbiol. 1990 Mar;31(3):195–201. doi: 10.1099/00222615-31-3-195. [DOI] [PubMed] [Google Scholar]
  23. Zollinger W. D., Mandrell R. E. Type-specific antigens of group A Neisseria meningitidis: lipopolysaccharide and heat-modifiable outer membrane proteins. Infect Immun. 1980 May;28(2):451–458. doi: 10.1128/iai.28.2.451-458.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zollinger W. D., Moran E. E., Connelly H., Mandrell R. E., Brandt B. Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping. Infect Immun. 1984 Oct;46(1):260–266. doi: 10.1128/iai.46.1.260-266.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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