Northern blot analysis of total RNA isolated from carnation flowers at different stages of post-harvest development and carnation leaves. Each lane contained 10 μg of RNA. Upper panels, RNA blots probed with carnation lipase cDNA; lower panels, agarose gels of fractionated RNA. (A) Lanes 1–4, flowers at stages I–IV of post-harvest development, respectively. (B), lanes: 1, stage II flowers; 2, stage II flowers treated with ethylene; 3, a mixture of senescing and nonsenescing leaves from stems bearing flowers at stage II of development; 4, leaves from stems bearing flowers at stage II of development that were treated with ethylene.