Table 2.

GC measurements of the lipolytic acyl hydrolase activity of maltose-binding protein and lipase fusion protein expressed in E. coli and purified by amylose column chromatography

Substrates	Products, μg/mg protein*
		Maltose-binding protein	Lipase fusion protein
Tri-linolein†	Linoleic acid (18:2)	15.9 ± 0.75	33.4 ± 1.58
Soybean phospholipid‡	Palmitic acid (16:0)	ND§	4.80
	Stearic acid (18:0)	ND	9.68
	Linoleic acid (18:2)	ND	5.80
Dilinoleylphosphatidylcholine‡	Linoleic acid (18:2)	ND	20.0

^*Reaction was allowed to proceed for 2 hr and was not continuously linear over this period. 

^†Means ± SE for n = 3 replications are shown. 

^‡Single experiment. 

^§ND, not detectable.