Table 3.
Predicted new TAS
| Toxin (T) | Antitoxin (AT) | Comment |
| MNT | HEPN | MNT – minimal nucleotidyltransferase, possible toxin; HEPN – possible substrate binding domain; Structure solved (MNT: 1no5 and HEPN: 1o3u and 1jog). Molecular mechanism unknown. |
| PIN | COG2442 | Structure of AT is solved (PDB:2ga1):DNA/RNA-binding 3-helical bundle. |
| PIN | COG2880 | Structure of AT is solved (PDB:2nwt); related to AbrB superfamily |
| PIN | COG1753 | AT – RHH (RHH); Specific for archaea |
| PIN | MerR | AT: truncated MerR |
| COG4679 subfamily | COG5606 HTH | AT – predicted HTH domain; T – predicted RelE superfamily protein |
| RelE | MJ1172 RHH | Specific for methanogens; AT – predicted RHH superfamily protein |
| MazF | XF1863 | No prediction for AT |
| Fic/Doc | YhfG | AT – is predicted DNA-binding protein; Specific for enteroproteobacteria |
| SMa0917 subfamily | PHD | T – predicted MazF superfamily protein; Molecular mechanism is likely the same as for MazF toxin |
| COG2929 |
COG5304 and COG3514 Families |
AT: predicted RHH family protein; Molecular mechanism unknown. |
| DUF397 | Xre/cro HTH | T – no prediction; molecular mechanism unknown. |
| COG2856 | Xre/cro HTH | T – predicted Zn-dependent protease. Often fused to AT domain. Frequent association with RelE and PIN toxins |
| COG5654 | COG5642 subfamily | AT – xre family HTH; T – RES domain; Molecular mechanism unknown. |
| YgiU/MqsR | Xre/cro HTH | T: motility quorum-sensing regulator mqsR [75] |
| GNAT | Xre/cro HTH | The closest characterized GNAT family acetyltrasferase is involved in antibiotic resistance [88] |
| GNAT | RHH | T – is GNAT family acetyltrasferase |
| Bro | Xre/cro HTH | |
| COG3832 | ArsR-like HTH | T – Cyclase/dehydratase family protein. (PDB: 1xuv) START domain superfamily |
New toxins and antitoxins predicted in this work are shown in bold type