Figure 4.

Southern hybridization of restriction enzyme digested psittacciform genomic DNAs. Genomic DNAs isolated from four psittaciform taxa (kaka, kea, kakapo and galah) were digested with four different restriction enzymes and used for Southern hybridization with three DIG-labeled probes generated from galah partial OR coding sequences: (A) probe Gal-A [GenBank: EU599486], (B) probe Gal-B [GenBank: EU599487] and (C) probe Gal-C [GenBank: EU599488]. 42°C hybridization temperatures were used for all results shown. Abbreviations: T, TaqI; P, PstI; E, EcoRI; H, HindIII; DIG, digoxigenin, Gal, galah. Approximate positions of the size standards (kb) are indicated. Note that each figure was generated by splicing together images from two separate blots (see Methods for details).