Table 1.

Protocol of experiments for generating the model

Sequence Number	Number of Cells (× 105)*	Amount of plasmid (μg) *	Amount of LipofectAMINE (μL) *	Expression ratio of GFP %	Fitted Values %
A	11.0	100.50	80.88	64.74	64.50
B	21.1	50.25	40.56	45.58	45.72
C	31.2	140.70	111.12	68.37	68.06
D	41.3	20.10	131.28	19.60	20.25
E	51.4	120.60	10.32	30.61	31.04
F	61.5	70.35	60.72	56.16	56.09
G	71.6	80.40	151.44	75.24	74.80
H	81.7	40.20	101.04	48.78	48.85
I	91.8	150.75	50.64	59.19	59.06
J	101.9	30.15	20.40	38.23	38.51
K	112.0	110.55	141.36	82.53	81.94
L	122.1	10.05	70.80	13.49	14.26
M	132.2	130.65	90.96	71.67	71.29
N	142.3	90.45	30.48	51.96	51.97
O	152.4	60.30	121.20	61.85	61.67

*: In these independent variables, the numbers outside of round brackets refer to serial numbers of their levels, and the numbers inside of round brackets refer to the actual dosages.