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. 2008 Aug 29;283(35):24274–24284. doi: 10.1074/jbc.M709971200

FIGURE 2.

FIGURE 2.

Cell penetration of MCab-Strep-Cy5 and MCab K20A-Strep-Cy5 in wild-type and HSPG mutant CHO cells. A, dose-dependent cell penetration of wild-type MCab-Strep-Cy5 in wild-type and mutant CHO cells, as assessed quantitatively by FACS. Results are from a representative experiment. The indicated concentrations are for streptavidin-Cy5 (from 10 nm to 2.5 μm). Data were fitted by a sigmoid equation providing the following half effective concentrations: PC50 = 0.46 ± 0.01 μm (wild-type CHO), 0.56 ± 0.01 μm (pgsD-677), and 0.71 ± 0.01 μm (pgsB-618). a.u., absorbance units. B, comparative cell penetration of 1 μm MCab-Strep-Cy5 and MCab K20A-Strep-Cy5 in CHO and CHO mutant cell lines. Results are from a representative experiment of n = 3. Values are normalized with mean fluorescence intensity of wild-type CHO cells.