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. 2009 Apr 29;76(1):105–112. doi: 10.1124/mol.108.053744

Fig. 1.

Fig. 1.

Block of osmotic water permeability of AQP4-expressing X. laevis oocytes by extracellular bumetanide but not torsemide. Swelling rates were standardized to the mean rate of swelling for oocytes with untreated AQP4 wild-type channels set as 100% from the same experiment to allow the compilation of data across batches of oocytes. Doses of bumetanide (Bum; a) and torsemide (Toro; b) were applied extracellularly in isotonic saline at the doses indicated on the x-axis in parentheses (micromolar) 1 to 2 h before measurement of swelling rate in 50% hypotonic saline without drug. The threshold dose of bumetanide needed for statistically significant block (p < 0.05) was 100 μM (analysis of variance, post hoc Student's t test). Torsemide had no statistically significant effect on swelling rate, ruling out an appreciable contribution of endogenous co-transporters that are sensitive to loop diuretic drugs. Data are the mean ± S.E.M.; n values are in italics above the x-axis.

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