Fig. 3.

Increased potency of block by intracellular delivery of bumetanide and furosemide via injection into AQP4-expressing oocytes. a, summary of the percentage block of swelling rates of AQP4-expressing oocytes after injection at 2 to 4 h before the swelling assay of a bolus volume (50 nl) of drug estimated to result in final intracellular concentrations of 0 to 100 μM (unpaired single swelling assays; see Materials and Methods for details). b, chemical structures of furosemide, bumetanide, and torsemide.