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. 2009 Apr 13;76(1):163–172. doi: 10.1124/mol.109.055855

Fig. 2.

Fig. 2.

Induction of caspase-dependent apoptosis by indolequinone 3 in pancreatic cancer cells. A, effect of indolequinone 3 treatment on apoptotic cell death. MIA PaCa-2 and BxPC-3 cells were treated with indolequinone 3 at various concentrations. Apoptosis was measured 24 h after 4-h drug treatment using annexin-PI staining in combination with flow cytometry analysis. B, effect of pretreatment with the pan-caspase inhibitor z-VAD-fmk on indolequinone 3-induced apoptosis. BxPC-3 cells were pretreated with 100 μM z-VAD-fmk for 30 min before indolequinone 3 treatment. Data represent mean ± S.D. of three independent determinations. C, induction of caspase activation by indolequinone 3 in MIA PaCa-2 and BxPC-3 pancreatic cancer cells. The cleaved forms of caspase 3, 7, 8, and 9 were detected in both cell lines using immunoblot analysis 24 h after 4-h drug treatment. Staurosporine (STAU) treatment (500 nM) was included as positive control for caspase activation. Immunoblot shown was representative of at least three independent experiments.