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In vitro-translated p70S6K is cleaved by caspase-3. EE-tagged p70S6K cloned into pcDNA3 was synthesized with the T7 Quick TNT kit (Promega) in the presence of [35S]Met and incubated with or without human recombinant caspase-3 as described in the “Experimental Procedures.” The reaction products were separated by SDS-PAGE and analyzed by autoradiography. Arrows indicate cleaved p70S6K bands. The results are representative of three separate experiments.
