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. Author manuscript; available in PMC: 2009 Jun 24.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 Jun 27;107(3-5):131–139. doi: 10.1016/j.jsbmb.2007.05.031

FIGURE 5. Over-expression of COBRA1 reduces the skipping/inclusion ratio of androgen-dependent alternative splicing.

FIGURE 5

COBRA1 and AR were co-expressed with the MMTV-CD44 mini-gene cassette in HEK293T cells. The transfected cells were treated with 1 nM R1881 for 24 hours before harvesting for alternative splicing assay. Two major products of CD44 mini-gene resulted from alternative splicing were designated as inclusion and skipping, respectively. The minor splicing product with one exon inclusion is also indicated in the figure, but not considered in the calculation of the skipping/inclusion ratio. The skipping/inclusion ratio was calculated by averaging two independent transfections in each assay. The diagram indicates various splicing events. The open and solid boxes in the diagram refer to the variable and invariable exons, respecitively. The arrows indicate the positions of the primers used in the PCR reactions.