Fig. 1.

A, B10.TCRδ−/− female mice frequently develop corneal opacities. Examples of B10.TCRδ−/− females with varying degrees of corneal opacity are shown, along with an unaffected eye for comparison (upper left panel). B, Overall percentage of mice with keratitis among different strains, determined by gross examination. Mice of mixed ages, all 7 weeks of age or older, were included. Total numbers of males (M) and females (F) that were examined are indicated for each strain. C, Hematoxylin/eosin stained sections of corneas from mice with opaque eyes. C1, Low magnification to show the overall structure of a normal B10 mouse eye. C2, Higher magnification of the normal cornea shown in C1. C3, Cornea of a B10.TCRδ−/− mouse showing slight opacity and a reddish cast to the eye. C4, Cornea of a B10.TCRδ−/− mouse with slightly greater corneal opacity and a reddish eye. C5, Cornea of a B10.TCRδ−/− mouse with severe opacity and central thickening, due to a hypertrophied corneal epithelium. C6, From a severely keratitic eye; the outer epithelium appears to have been sloughed (note: C2–C6 are at the same magnification). D, High magnification of the cornea from a severely keratitic eye. E, Immunofluorescent staining of frozen section of keratitic B10.TCRδ−/− cornea stained with anti-TCRβ (red) and anti-CD4 (blue); double positive CD4 αβ T cells are pink. F, Immunofluorescent staining of frozen section of keratitic B10.TCRδ−/− cornea stained with anti-TCRβ (red) and anti-CD8α (blue); double positive CD8 αβ T cells appear pink. In both E and F, the autofluorescent background (green) has been deliberately enhanced to reveal the tissue structure.