Fig. 3.

SDS-PAGE of [³²P] Pi-labeled, gel-filtered platelets treated with thrombin in the absence or presence of drugs. Aliquots (500 µl) of [³²P]Pi-labeled, gel-filtered platelets were incubated with 50 µl of the drugs (30 µM, final concentration) or their solvents for 90 s at 37 °C, then 50 µl thrombin (0.45 U/ml, final concentration) or NaCl (0.15 M) were added to the samples, and incubation continued for another 6 min, then 150 µl of incubation mixture was immediately mixed with 75 µl 4× Laemli buffer as described in the “Materials and methods” section (thrombin-induced phosphorylation of 20-KDa MLC)