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. Author manuscript; available in PMC: 2009 Jun 24.
Published in final edited form as: Science. 2008 Oct 31;322(5902):703–709. doi: 10.1126/science.1160058

Fig. 4. Rates of spontaneous immortalization of primary aneuploid MEFs.

Fig. 4

(A) Cells were serially passed in a 3T3 immortalization assay (12) and the number of population doublings are shown as a function of number of passages.

(B) The mean number of passages (passage) until immortalization was calculated as described (12). The standard deviations are shown in the column to the right. P-values are given in the last column. Note that the average number of passages until immortalization for the Ts16 cell lines does not include the Ts16 cell line that failed to immortalize. Note that for Ts19 two of the three immortalization assays were performed in independent experiments with cells obtained from the same embryo.