β-L-D4A inhibited the expression of EGFP in HepG2 cells transfected with pEGFP-Sp, as determined by FACS analysis. A: HepG2 cells not transfected with pEGFP-Sp, not treated with drug, the percentage of EGFP-positive cells was 0; B: HepG2 cells transfected with pEGFP-Sp, not treated with drug, the percentage was 21.42%; C: HepG2 cells transfected with pEGFP-Sp, treated with Lamivudine at 1 μmol/L, the percentage was 21.14%; D-G: HepG2 cells transfected with pEGFP-Sp, treated with β-L-D4A at various concentrations (0.08, 0.4, 2, 10 μmol/L), the percentages were 18.76%, 17.31%, 15.53%, 13.65% respectively.