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. Author manuscript; available in PMC: 2009 Jun 25.
Published in final edited form as: J Cell Sci. 2004 Nov 9;117(Pt 25):6019–6030. doi: 10.1242/jcs.01400

Figure 5.

Figure 5

The intra-S phase checkpoint reduces Cdc45 and PCNA on chromatin.

(A) Sperm nuclei were incubated at 10 ng DNA/μl in interphase extract supplemented with various concentrations of aphidicolin minus (left panel) or plus 5 mM caffeine (right panel). Chromatin was isolated and immunoblotted for XCdc45 and Rad17. (B) Sperm nuclei were incubated at 10 ng DNA/μl in interphase extract supplemented with different combinations of 15 μM aphidicolin and/or 5 mM caffeine. At the indicated times, chromatin was isolated and immunoblotted for XCdc45 and PCNA.