Figure 3.

Immunodepletion experiments with anti-p140 and anti-p155/140. The autoradiogram shows the results of 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis of immunoprecipitates, using different anti-p140–positive juvenile DM sera (lanes 1, 2, 5, and 6) and anti–p155/140–positive juvenile DM sera (lanes 3 and 4). Immunoprecipitation was performed with ³⁵S-methionine–labeled K562 cell extracts predepleted with either reference anti-p140–positive juvenile DM serum or reference anti-p155/140–positive juvenile DM serum. See Figure 2 for definitions.