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. 2009 Jun 15;23(12):1461–1473. doi: 10.1101/gad.517009

Figure 5.

Figure 5.

Zic1 controls cyclops expression and interferes with Nodal signaling. (A–F) Dorsal view, rostral to the bottom. (A–D) 11s. (E–F′) 1s. (A) Knockdown of Zic1 cannot block smad2CA mRNA-induced shh expression (arrow). (B) In Zic1 morphants, shh is down-regulated in the anterior forebrain (arrow). (C) Smad2CA mRNA injection causes induction of shh expression (arrow). (D) Shh expression in the anterior forebrain of CoMO-injected embryos (arrow). (E–F′) Cyc expression is strongly down-regulated in the anterior ventral forebrain of Zic1 morphants (arrow; dashed line indicates level of sections). (G) A Nodal luciferase reporter construct indicates reduced Nodal signaling in Zic1 morphants at the 1–2-somite stage. Error bars indicate SEM. Asterisk indicates statistical significance; t-test, (*) p < 0.05. (RLU) Normalized relative light units; (s) somite stage.