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. 2009 Jun 15;23(12):1393–1398. doi: 10.1101/gad.519409

Figure 3.

Figure 3.

The β-cat-binding site and the TM are essential for Xcad-11 function. (A) Xcad-11 constructs for reconstitution. (S) Signal peptide (green); (EC) extracellular domains (orange); TM (red); (p120/β-cat) p120-binding site (blue) or β-cat-binding site (dark red); intracellular domain (yellow). (B) Whole-mount ISH. Lateral view of CNC at stage 26, analyzed by expression of twist. (Left column) Noninjected side (nis). (Right column) Injected side (is). Embryos injected with Xcad-11MO (2 pmol) together with 50 pg of Δe or ΔeΔp120 showed twist signal in pharyngeal pouches, while coinjection of 75 pg of Δc, 50 pg ΔeΔβ-cat, ΔeΔTM, or ΔeΔIMD did not. (C) Statistics for reconstitution. In the case of Xcad-11MO injection, only 28% of embryos showed normal migration. Coinjection of 75 pg of FLXcad-11 resulted in 82%, 75 pg of Δe in 88%, and 75 pg of ΔeΔp120 in 82% of embryos with CNC present in pharyngeal pouches; in the case of Δc, ΔeΔβ-cat, ΔeΔTM, and ΔeΔIMD coinjection, only 30%, 27%, 28%, and 52%, respectively. (D) CNC explants. Coinjection of FLXcad-11, Δe, or ΔeΔp120 with Xcad-11MO restored formation of lamellipodia and filopodia in CNC; ΔeΔβ-cat coinjection showed blebbing. Bar, 10 μm.