A. Diabetes disease onset detected by urinary glucose test. There was no urine glucose detected in any animal group at the age of 8 weeks. At 16 weeks of age, 8 animals in the water-fed group tested positive for urine glucose; 3 animals in the EGCG-fed group were positive. At 22 weeks of age, only 3 out of 12 animals in the water-fed group were negative for urine glucose, while 7 out of 12 in the EGCG-fed animals were negative. B. Total serum anti-nuclear antibody determination by ELISA. Serum total anti-nuclear antibody concentration in each NOD mouse was measured by the Mouse Anti-Nuclear Antibodies (ANA) ELISA Kit at the age of 8 (n=12), 16 (n=10) and 22 weeks (n=9). Values of each bar represent absorption measurements for each group. Y-error bars represent SEM. At the age of 22 weeks, serum total anti-nuclear antibody levels are significantly lower in EGCG-fed animals than water-fed animals (two-tailed student t-test analysis, p<0.005).