(A) Representative traces showing NANC-induced relaxation in cavernosal segments from control (top) and TNF-α-infused (bottom) mice; (B) Frequency-responses curves elicited by EFS (NANC nerves stimulation) and (C) acetylcholine concentration-response curves in cavernosal strips pre-contracted with PE (10−5 M), from control (●) and TNF-α-infused (■) mice. Experimental values of the relaxations induced by EFS (n=9 and 10, respectively), and acetylcholine (n=7 and 10, respectively) were calculated relatively to the maximal changes from the contraction produced by PE in each tissue, which was taken as 100%. Data represent the mean ± SEM of n experiments. *, P<0.05 vs. control.