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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4043–4051. doi: 10.4049/jimmunol.181.6.4043

FIGURE 5.

FIGURE 5

Macrophages (MΦ) acquire B cell-derived membrane and/or intracellular proteins along with Ag. BJAB cells were labeled with CTO and pulsed with Ag. Macrophages were labeled with CTFR and then cocultured for 18 h with the following Ag-pulsed B cell populations: no Ag (gray curve), nonspecific Ab (dotted curve), and anti-Ig (aIgG/M (F(ab)2; filled curve). Cells were harvested and analyzed by flow cytometry. A, B cell specific fluorescence is transferred to macrophages. Level of the B cell-derived label (CTO) was determined for the FSC/SSC-gated population corresponding to macrophages (CTFR). B, Macrophages positive for B cell-derived fluorescence acquire the highest amount of transferred Ag. Levels of transferred Ag (AF488) were assessed for the double stain (CTO+CTFR+; upper panel) gated macrophage population and for those that were single stain (CTFR+ alone; lower panel).