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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4043–4051. doi: 10.4049/jimmunol.181.6.4043

FIGURE 7.

FIGURE 7

Expression of SR-A is required for Ag transfer to differentiated THP-1 cells. A, Differentiated THP-1 cells acquire more transferred Ag than those left undifferentiated. THP-1 cells were cultured in the absence or presence of PMA for 24 h before being cocultured with B-LCL cells pulsed with either nonspecific Ab or anti-Ig (aIgG/m F(ab)2). Cells were harvested and stained for flow cytometry with Abs to CD19 and CD14. MFI values for transferred Ag (AF488) are given for the CD19−CD14+-gated populations from five independent experiments (*, p = 0.00003). B, SR-A mediates Ag transfer to differentiated THP-1 cells. PMA-differentiated THP-1 cells were left untreated (UT) (filled curve) or pretreated for 1 h with anti-SR-A (aSR-A; open curve with heavy line)- or anti-SR-B (aSR-B; dotted curve)-blocking Abs before being cocultured for 18 h with B-LCL cells pulsed with anti-Ig. As a negative control, PMA-differentiated THP-1 cells were left untreated and cocultured with B cells pulsed with nonspecific Ab (light gray-shaded curve). Cells were stained for flow cytometry and the levels of transferred Ag were assessed for the CD19−CD14+-gated population.