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Primary human B cells transfer Ag to macrophages. Primary human B cells were isolated from PBMC and pulsed for 60 min with the following: no Ag (gray-shaded curve), nonspecific Ab (dotted curve), or anti-Ig (open curve with heavy line). These cells were then cocultured with either undifferentiated (upper panel) or PMA-differentiated (lower panel) THP-1 cells for 18 h before being harvested for flow cytometry. Levels of transferred Ag were assessed for the CD19−CD14+-gated population.
