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. Author manuscript; available in PMC: 2009 Jun 25.
Published in final edited form as: Tetrahedron Lett. 2007 Jul 16;48(29):5107–5110. doi: 10.1016/j.tetlet.2007.05.082

Figure 1.

Figure 1

Chiral HPLC profile of the enzymatic hydrolysis of racemic Boc-Alloc-Ncy(Tmob)-OCH3 (5d). Reaction progress (a) initial, retention time (tR) for racemic ester is 30.34 min and 30.96 min (b) after 24 h, tR for resolved Alloc-Ncy(Tmob)-OH is 25.32 min and tR for unreacted Alloc-DNcy(Tmob)-OCH3 is 30.31 min. The HPLC assay conditions for the chiralcel® OD-RH™ reversed-phase column (0.46 cm × 15 cm): buffer A, 0.1% TFA in H2O, buffer B, CH3CN in A; gradient elution from 10% B – 70% B in 30 min and then 95% B in 2 min at a flow rate of 0.5 mL/min. UV detection, 0.1 AUFS at 210 nm.