Fig. 4.

Transcript level of CHS in the buds of male-sterile (S) and fertility-restored (F) radish plants detected by real-time RT-PCR. Total RNA was isolated from buds (<2 mm) of each plant. ‘MS-G’ and ‘60-W’ are male-sterile with orf138; ‘MS-G’ × ‘Tomioka’, ‘MS-G’ × ‘Iwasaki’ and ‘60-W’ × ‘Comet’ are F₁ plants that produce fertile pollen because of the Rf gene derived from the pollen parents (‘Tomioka’, ‘Iwasaki’ and ‘Comet’).