Table 1.
Real-time RT-PCR analysis of expression of flavonoid biosynthetic genes in buds of ‘MS-G’ (sterile) and ‘UC-G’ (fertile) radish lines
| Relative transcript* |
|||||
|---|---|---|---|---|---|
| Cultivar | C4H | 4CL | CHS | CHI | F3H |
| ‘MS-G’ | 0·56 ± 0·06 | 0·42 ± 0·03 | 0·10 ± 0·05 | 0·46 ± 0·01 | 1·17 ± 0·06 |
| ‘UC-G’ | 0·51 ± 0·12 | 0·36 ± 0·05 | 4·29 ± 1·04 | 0·60 ± 0·05 | 1·34 ± 0·17 |
* The relative transcript level was calculated as target quantity divided by the quantity of a constitutive control gene, Actin.
Abbreviations: C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate coA ligase; CHS, chalcone synthase; CHI, chalcone isomerase; and F3H, flavonol 3-hydroxylase. Each clone-specific primer set was designed from the corresponding gene in radish. Total RNA was isolated from buds (<2 mm) of ‘MS-G’ and ‘UC-G’ lines. Values are mean ± s.e. calculated from four technical replicates of a total of two independent experiments.