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Transcript levels of CHS at various stages of radish anther development, assessed by real-time RT-PCR in ‘MS-G’ (sterile) and ‘UC-G’ (fertile) lines
| Bud length |
|||
|---|---|---|---|
| Cultivar | 2–3 mm | 3–4 mm | 5–6 mm |
| ‘MS-G’ | 0·07 ± 0·02 | 0·02 ± 0·02 | 0·04 ± 0·01 |
| ‘UC-G’ | 5·0 ± 1·8 | 8·6 ± 2·6 | 0·6 ± 0·6 |
Values are mean (± s.e.) relative transcript levels from three independent anther samples of total RNA, calculated as target quantity divided by the quantity of a constitutive control gene, Actin.
