Fig. 7.

(A) Confirmation and characterization of DOG10, DOG11and DOG12 in the promotion of germination by light using heterogeneous inbred families (HIFs). Seeds were stored at 25 °C for 180 d (DOG10), 210 d (DOG11) and 300 d (DOG12), and then incubated for 3 d at 7 °C followed by a pulse of R or FR, or kept in darkness (D). Seeds were harvested simultaneously from plants grown in identical environmental conditions, and therefore the HIFs are directly comparable at the same marker. Values are means of 6–18 replicates ± s.e. Only significant differences between means are shown: ***, P < 0·01; **, P < 0·05 (Student's t-test). HIFs carried on Bay and Sha alleles at the indicated marker are shown. (B) Graphical representation of the genotypes of the parental lines Bay, Sha and the 5 HIFs segregating at the marker of interest as Bay (black) or Sha (white), and homozygous elsewhere (grey).