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. 2009 Jun 1;119(7):1974–1985. doi: 10.1172/JCI33816

Figure 2. Construction and function of the Ad-RAD50 vector.

Figure 2

(A) A 326–base pair mutant RAD50 hook fragment from the wild-type RAD50 gene was cloned into a recombinant adenovirus vector to construct Ad-RAD50. The amino acid sequence of the integrated construct (residues 631–739) is highlighted in black, with the zinc hook region marked in yellow. PA, polyadenylation site. (B) Both the wild-type RAD50 protein and the mutant RAD50 protein were detected by Western blot in JHU012 tumor cells that were infected with Ad-RAD50 adenovirus. Lane 1, MagicMark Western Protein Standard; lane 2, JHU012 cells with no treatment; lane 3, JHU012 cells infected with Ad-RAD50.