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. 2009 Jun 8;119(7):1952–1963. doi: 10.1172/JCI37506

Figure 1. Vector constructs.

Figure 1

(A) For the GFP-MGMT* SIV-based lentiviral vectors, expression of a GFP-MGMT* fusion gene was driven by the promoter activity of the MSCV LTR. (B and C) For MGMT*-P2A-GFP (B) and MGMT*-P2A-YFP (C) SIV vectors (referred to herein as MGMT*-GFP and MGMT*-YFP, respectively), P2A was used to generate a multicistronic expression cassette inserted in the pCL20cSLFR MSCV-GFP backbone. The primer pairs (M3, M5, Y3, Y5, G3, and G5) used for construction of the vectors are described in Methods. (D) For MGMT*-P2A-HOXB4-T2A-GFP SIV vectors (referred to herein as MGMT*-HOXB4-GFP), T2A was used to generate a multicistronic expression cassette inserted in the pCL20cSLFR MSCV-GFP backbone. MGMT*, HOXB4, and GFP were amplified and 2A-tagged from their respective cDNAs using primer pairs M5, M3, H5, H3, G5, and G3 as described in Methods.

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