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. 2009 Jun 22;119(7):2062–2073. doi: 10.1172/JCI38714

Figure 2. CCR7-dependent selective loss of DCs from the nonpregnant uterus following LPS stimulation.

Figure 2

(A) Flow cytometric analysis of uterine leukocytes 28 hours after intravenous LPS injection. The number of cells shown for each mouse is normalized to a fixed number of CD45– non-rbc, which we used as estimates of uterine parenchymal cell number. CD86 expression levels by those few uterine DCs remaining in LPS-treated wild-type mice varied between individual mice. (B) Cell numbers for LPS- and control PBS-treated mice were calculated by flow cytometry using the gates shown in A and were normalized to CD45– non-rbc. Data show mean ± SEM of n = 6–7 mice per group, compiled from 3 independent experiments. (C and D) Representative sections of uteri from PBS- (C) and LPS-treated (D) mice, immunostained with anti-MHCII (green) and anti-F4/80 (red) antibodies. DCs are pure green (MHCII+F4/80–) cells. Color intensities in both images were subjected to the same set of nonlinear adjustments so that the cells would be visible at low magnification. Scale bar: 0.5 mm. (E) Histomorphometric quantification of DC densities in the myometrium and endometrium of PBS- and LPS-treated mice (mean ± SD; n = 3 mice per group).