Figure 5. Defective EMT in Islet1^Cre/+;DNMAML mutants is rescued by rFgf8.

(A–D) Representative images of outflow explants taken 48 hours (24 hours after treatment with rFgf8 or vehicle) after plating. Control explants treated without (A) or with (B) rFgf8 underwent EMT and invasion into the collagen gel. (C) Islet1^Cre/+;DNMAML explants displayed deficient EMT. (D) Addition of rFgf8 to mutant explants rescued EMT. (E–H) Similar results were seen at 72 hours. (I–K) Quantitative analysis was performed on samples pooled from 3 independent experiments at 24, 48, and 72 hours. Error bars represent 1 standard deviation. Control genotype was Islet1^+/+;DNMAML. Scale bars: 100 μm.