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. 2009 Jun 22;119(7):2074–2085. doi: 10.1172/JCI38940

Figure 4. Cauda-retrieved spermatozoa of Gpx5–/– animals suffer oxidative damages.

Figure 4

(A) Immunodetection of the nuclear adduct 8-oxodG in cauda epididymidis-retrieved spermatozoa preparations from 6-month-old WT, KO, or hydrogen peroxide–treated WT male mice. Images are representative of several distinct detections. (B) Histograms showing the percentage of 8-oxodG–positive spermatozoa in cauda epididymidis-retrieved spermatozoa preparations, from 6-month-old WT, KO, and positive control (WT spermatozoa treated with hydrogen peroxide) male mice. Data are mean ± SEM. n = 3. **P < 0.05.