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. Author manuscript; available in PMC: 2009 Jun 25.
Published in final edited form as: J Neuroimmunol. 2008 Nov 21;206(1-2):14–21. doi: 10.1016/j.jneuroim.2008.10.003

Fig. 7.

Fig. 7

Effect of APOBEC3G-specific siRNA on APOBEC3G expression and MLV-env-psedoutyped-HIV-1 replication in NT2-N and U87-MG cells. (A) Down-regulation of APOBEC3G mRNA expression in NT2-N and U87-MG cells by the siRNA against APOBEC3G. NT2-N and U87-MG cells were transfected with APOBEC3G-specific siRNA or siRNA-Ctl for 24 h. Total RNA was extracted for analyzing APOBEC3G mRNA expression by the real-time RT-PCR. (B) Effect of APOBEC3G-specific siRNA on MLV-env-psedoutyped-HIV-1 replication in NT2-N and U87-MG cells. The cells were transfected with the siRNAs as described above and then infected with MLV-env-psedoutype-HIV-1. NT2-N or U87-MG cells were collected for luciferase activity 72 h postinfection. The data shown in both A and B is expressed as mean±SD of triplicate cultures, representative of three independent experiments (*, p<0.05; siRNA-A3G treated vs. siRNA-Ctl treated).